Detection of Minimal Residual Disease in Acute Lymphoblastic Leukemia
نویسندگان
چکیده
MRD diagnostics during the first three months of treatment has proven to be of high value in pediatric acute lymphoblastic leukemia (ALL), because of its potential to recognize subgroups that differ substantially in outcome. Consequently, MRD diagnostics is now being used for treatment intervention, both treatment intensification (including stem cell transplantation) and treatment reduction. However, the possibilities for recognition of MRDbased risk groups are dependent on the timing of the follow-up samples and the sensitivity of the applied MRD method. The currently available data indicate that two time points are needed for identification of both high-risk and low-risk patients and that a sensitivity and quantitative range of at least 10 are needed. Current 4-color flow cytometry can be used for identification of high-risk patients and the more sensitive PCR techniques can be used also for identification of true low-risk patients with very low relapse rates, who might profit from treatment reduction. The new development in standardized 8-color flow cytometry might well be able to compete with MRD-PCR methods.
منابع مشابه
Evaluation of Relationship between Minimal Residual Disease (MRD) and Relapse in Childhood Acute Lymphoblastic Leukemia (ALL) Using Quantitative Fluorescent PCR
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